Assemblage of Ortho Cleavage
نویسنده
چکیده
Genetic engineering is a powerful means of accclcrating the evolution of new biological activities and has coDsiderable potential for constructing microorganisms that can degrade environmental poUutants. Critical enzymes from five different catabolic pathways of three distinct soil bacteria have been combined in patq,.work fashion into a functional ortbo cleavage route for the degradation of methylphenols and methyl. benzoata. The new bacterium thereby evoJyed was able to degrade and grow on mixtures of chloroand methylaromatics that wen: toxic even for the bacteria that could degrade the individual components of the mixtures. Except for one enzymatic step, the pathway was fully regulated and its component enzymes were only synthesized in response to the prescncc of patbw.ay substrates.
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